cell proliferation assays primary human coronary artery ecs  (PromoCell)

Journal: ACS biomaterials science & engineering

Article Title: TiO 2 -Based Nanotopographical Cues Attenuate the Restenotic Phenotype in Primary Human Vascular Endothelial and Smooth Muscle Cells

doi: 10.1021/acsbiomaterials.9b01475

Figure Lengend Snippet: EC and SMC cell coverage on flat and NT surfaces. (A) EC and (E) SMC cell numbers on flat and NT surfaces were measured using a CyQUANT assay, which quantifies DNA content in cell lysates. (B) EC and (F) SMC area were quantified using fluorescence microscopy and ImageJ. Representative images of (C,D) ECs and (G,H) SMCs cultured on flat and NT surfaces are shown. Green: phalloidin. Blue: DAPI. Scale bar: 150 μm.

Article Snippet: Cell Culture and Cell Proliferation Assays Primary human coronary artery ECs and primary human coronary artery SMCs were purchased from PromoCell (Heidelberg, Germany).

Techniques: CyQUANT Assay, Fluorescence, Microscopy, Cell Culture

Journal: ACS biomaterials science & engineering

Article Title: TiO 2 -Based Nanotopographical Cues Attenuate the Restenotic Phenotype in Primary Human Vascular Endothelial and Smooth Muscle Cells

doi: 10.1021/acsbiomaterials.9b01475

Figure Lengend Snippet: Total FAK and pFAK were quantified by ELISA using cell lysates from (A,B) ECs and (C,D) SMCs cultured on flat and NT90 surfaces. Total FAK concentration was normalized to the total protein content. The pFAK concentration was normalized to the total FAK concentration. Data are expressed as mean ± SD.

Article Snippet: Cell Culture and Cell Proliferation Assays Primary human coronary artery ECs and primary human coronary artery SMCs were purchased from PromoCell (Heidelberg, Germany).

Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture, Concentration Assay

Journal: ACS biomaterials science & engineering

Article Title: TiO 2 -Based Nanotopographical Cues Attenuate the Restenotic Phenotype in Primary Human Vascular Endothelial and Smooth Muscle Cells

doi: 10.1021/acsbiomaterials.9b01475

Figure Lengend Snippet: Effect of NT topography on inflammatory response. (A) VCAM-1 gene expression in ECs. (B) SMC cell numbers when cultured on flat or NT90 surfaces in control media or under stimulation with 2 ng/mL TNFα, an inflammatory cytokine and known mitogen for SMCs. (C) MCP-1 secretion by SMCs cultured on flat or NT surfaces was measured in conditioned media using ELISA. Data are expressed as mean ± SD.

Article Snippet: Cell Culture and Cell Proliferation Assays Primary human coronary artery ECs and primary human coronary artery SMCs were purchased from PromoCell (Heidelberg, Germany).

Techniques: Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: ACS biomaterials science & engineering

Article Title: TiO 2 -Based Nanotopographical Cues Attenuate the Restenotic Phenotype in Primary Human Vascular Endothelial and Smooth Muscle Cells

doi: 10.1021/acsbiomaterials.9b01475

Figure Lengend Snippet: Effect of varying NT diameter on EC and SMC response. Cell growth rates on flat and NT surfaces using (A) ECs and (D) SMCs. Significant values are represented as follows: x NT30; # NT50; *NT90. One symbol: p < 0.05; two symbols: p < 0.01. (B) Relative VCAM-1 mRNA expression in ECs cultured on flat and NT surfaces. Values are normalized to mRNA expression on flat surfaces. (C) SMC cell numbers cultured on flat and NT surfaces in control media and in media containing 2 ng/mL TNFα. (E) MCP-1 secretion by SMCs cultured on flat and NT surfaces. Note: data shown here for flat and NT90 surfaces are identical as those in previous figures. They are shown here as a reference for comparison purposes.

Article Snippet: Cell Culture and Cell Proliferation Assays Primary human coronary artery ECs and primary human coronary artery SMCs were purchased from PromoCell (Heidelberg, Germany).

Techniques: Expressing, Cell Culture